BAX anticorps (AA 3-16)

N° du produit ABIN3025801

Cet anticorps anti-BAX est un anticorps Souris Monoclonal détectant BAX dans WB, IF, FACS et IHC (p). Adapté pour Humain et Singe.

Aperçu rapide pour BAX anticorps (AA 3-16) (ABIN3025801)

Antigène: BAX (BCL2-Associated X Protein (BAX))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BAX est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM336

Détail du produit anti-BAX anticorps

Épitope: AA 3-16

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogène: Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.

Isotype: IgG1

Information d'application

Indications d'application: The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-Bax antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur BAX

Antigène: BAX (BCL2-Associated X Protein (BAX))

Autre désignation: Bax

Sujet: Recognizes a protein of 21  kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Pathways: Signalisation p53, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response